Observation and analysis of cells in a stromal extracellular matrix (ECM), for example, collagen and matrigel, are essential for a full understanding of cell behavior. ECM mimics the structure and composition of the in vivo environment and affects many cellular properties, particularly adhesion, migration, and differentiation.
Thanks to the coherence-gating effect, a unique feature enabled by Telight Q-Phase, the QPI image quality is not impaired by the presence of the disturbing matrix. Therefore, the Q-Phase allows precise cell observation and analysis in scattering media.
QPI of human breast adenocarcinoma cell line MCF-7 in collagen
Analysis of cell mass during cell migration in the extracellular matrix is possible with Q-Phase
Human fibrosarcoma cell movement inside fibril of collagen gel | The distribution of cell mass during migration was studied in mesenchymal and amoeboid types
QPI of human fibrosarcoma cells in collagen
The patented design of Q-Phase allows high-quality QPI in turbid media
APODOX nanomaterial induced cell death in LNCap cell line | Magnification 10x
The patented design of Q-Phase allows high-quality OPI even in a semi-transparent medium
Colorectal cancer cells in 0,15% phospholipid emulsion | Magnification 20x | Collakova, J. et al.: Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion, Journal of Biomedical Optics, 2015
Quantitative Phase Imaging
Q-Phase is a patented type of holographic microscope with high detection sensitivity
Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.