Super-resolution microscopy

With the development of new techniques, fluorescence optical microscopy can now help biologists decipher the dynamics of biological processes at the scale of a few tenths of a nanometer.

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LiveCodim, is a super-resolution solution for every lab. Book a demo with us and see how easy it is to upgrade your confocal microscope to a super-resolution microscope.

LiveCodim uses a unique patented laser beam shaper. Our technology perfectly fits the needs of biologists in super-resolution and is adapted for living samples, to visualize cellular and molecular dynamics in high resolution.

COS-7 cells cytoskeleton |Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Publications

J. Vargas, et al.

The Wnt/Ca2+ pathway is involved in interneuronal communication mediated by tunneling nanotubes

EMBO Journal, 2019 Download PDF

Maarifi, G., Fernandez, J., Portilho, D.M., et al.

RanBP2 regulates the anti-retroviral activity of TRIM5α by SUMOylation at a predicted phosphorylated SUMOylation motif

Communications Biology, 2018 Download PDF

Garita-Hernandez M., et al.

Optogenetic light sensors in retinal organoids

Frontiers in Neuroscience, 2018

Getz A.M., Xu F., Visser F., et al.

Tumor suppressor menin is required for subunit-specific nAChR Alpha5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons

Scientific Reports (Nature), 2017 Download PDF

Products

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is the universal 3-in-1 imaging platform for wide-field, confocal and super resolution microscopy. It is designed to interface with any standard fluorescence microscope and is the solution for live cell imaging with high resolution and low phototoxicity.

Telight

Super-resolution microscopy

COS-7 cells cytoskeleton |Actin cytoskeleton is visualized using Sir-Actin marker

Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Mitochondrial dynamics in MDCK cells | Marker: Mitotracker-Orange | Mitochondria movements are visualized with 1 image every 5 seconds

Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein involved in linkage of integrin to the actin cytoskeleton

COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker

Z-stack visualization of COS-7cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Z-stack were acquired every 1µm on 10µm range

3D visualization of COS-7 cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Color code used in the lower panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Comparison of widefield and super-resolution visualization of fixed COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Confocal (made with LiveCodim) visualization of COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Comparison of widefield, confocal, and super-resolution of microtubule visualization in COS-7 cells | Marker: Anti-beta tubulin antibody and TMR coupled secondary antibody

3D visualization of mitotic spindles in COS-7 cells| Cells are labeled for microtubules (TMR- Red). Color code used in the right panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

COS-7 cell during cell division (metaphase) | Visualization of actin cytoskeleton (SiR-Actin, purple), mitochondria outer membrane (TOM-20 immunolabelling, green), and microtubules (TMR- Red)

Maximum Intensity projection visualization of COS-7 cell during cell division (metaphase) | Marker: actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). Images show maximum intensity projection of 10 Z-stack done every 1µm on a range of 10µm

COS-7 cell division | 3D visualization of actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). On the right panels, color code used represents the thickness of the sample with blue corresponding to 0 and yellow to 9.6µm (step: 1µm)

Widefield, confocal, and super-resolution visualization of MDCK cells expressing fluorescent Vinculin (green) and labeled with mitotracker-Orange (red)

2 color dynamic of mitochondria and Vinculin in MDCK cell | Cell expressing fluorescent Vinculin were labeled using mitotracker-Orange and images every 2min for 10 min.

Dynamic of mitochondria in MDCK cells Cells are labeled with mitotracker-orange (1 image acquisition every 1 min for 30 min)